Overview

Laboratory Technician for the Research Group “RNA-based Regulation of Gene Expression”

Department

Peter Lukavsky Research Group – Central European Institute of Technology

 

Research Group Leader: Peter Josef Lukavsky, Ph.D.

 

Deadline

31 December 2018

Start date

Negotiable

 

A position for a laboratory technician is available in the research group “RNA-based regulation of Gene Expression” which is part of Structural Biology program at CEITEC, Masaryk University (MU). We study disease-related biological processes by investigating protein structures and their interactions with other proteins, RNA or small molecule inhibitors using biomolecular NMR and related biophysical methods.

 

Job Description:

– Expression and purification of proteins

– Transcription and purification of RNAs

– Preparation of various media and buffers

– PCR and cloning of RNA and protein constructs

– Preparation of isotope-labelled nucleotides

– Binding studies using ITC, fluorescence anisotropy, EMSA,
and other biophysical techniques including data analysis

– Performance of NMR experiments to study small molecule interactions with proteins and RNA

– Implementing new techniques and projects

– Help incoming co-workers into a smooth start in the lab

 

Requirements:

– At least Master degree in molecular biology, biochemistry, biophysics, chemistry or other related fields of life-sciences

– Good knowledge of English (oral and written)

– Excellent interpersonal and communication skills

– High motivation, flexibility and self-driven interest to learn new things

– Reliability, team player, meticulousness

– Knowledge of “laboratory chemistry” – preparation of solutions with defined concentrations, adjusting pH, preparation of media for bacterial cultures etc.

– Computer literacy, MS word, Excel.

– Excellent experience with molecular biology, protein and RNA purifcation, biochemistry and NMR spectroscopy is a big plus

 

We offer:

– Interesting work in a top scientific team
– Stable base and excellent instrumental equipment of CEITEC MU
– employment benefits
– Salary based on qualification and experience; part time appointment possible

Location

Brno is an attractive, lively place to live and work with many cultural and recreational amenities.
Please send CV and a short motivational letter including: a) description of previous experiences b) technical skills and c) at least two contact references to Peter Lukavsky
(peter.lukavsky@ceitec.muni.cz). Candidates considered for the second round of interview will be informed via email.

Start date: Negotiable
Opened Until: 31.12.2018

I AM INTERESTED

Source: https://www.ceitec.eu/laboratory-technician-for-the-research-group-rna-based-regulation-of-gene-expression/j89